Untersuchungen zur genetischen Variabilität von Brassen-Populationen (Abramis brama) durch RAPD-PCR. (German)
Bartel, M., Kautenburger, R., Klein, R., and Müller, P.
(1998) Mitteilungen 20 (Oktober 1998): Institut für Biogeographie der Universität des Saarlandes; 1 - 16.
Investigations to the genetic variability of bream populations (Abramis brama) with RAPD-PCR
The knowledge of the genetic structure of populations is an important basis for comparability and adequate interpretation of environmental data. For the environmental specimen bank and the environmental samples stored above liquid nitrogen it is a substantial element of a qualitatively improved characterisation of samples. In the present work the genetic differentiation of spatially separated bream populations (Abramis brama) from the river Rhine were examined with RAPD-PCR. Additionally and in comparsion breams from the lake of Belau (Schleswig-Holstein) as well as other species like Blicca bjoerkna and Rutilus rutilus were included into the investigations. With the ROTH random primer kit 180 reproducible results could be obtained, which were also statistically evaluated by the method after LYNCH & MILLIGAN (1994). This method of estimation is able to correct the underestimated frequency of the zero-allele caused by the dominance of the RAPD markers. The calculated genetic diversity (H j) shows that the widespread Abramis brama is exeed in its genetic variability by sympatric occurring Rutilus rutilus. The genetic diversity between the examined populations (H jk), the genetic distance (D jk) and the population differentiation (F ST) do not indicate or show only weak differentiations between the spatially separated bream populations. The relatively high genetic similarity underlines the suitability of Abramis brama as indicator for accumulation and effects.